Antinociceptive effect of semi-purified petroleum ether partition of muntingia calabura leaves

Zainul Amiruddin Zakaria, Mohd Hijaz Mohd Sani, Arifah Abdul Kadir, Teh Lay Kek, Mohd Zaki Salleh

Research output: Research - peer-reviewArticle

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Abstract

Muntingia calabura L., Muntingiaceae, is a medicinal plant for various pain-related diseases.The aims of the present study were to determine the antinociceptive profile and to elucidate the possible mechanisms of antinociception of petroleum ether partition obtained from crude methanol extract of M.calabura leaves using various animal models.The antinociceptive profile of petroleum ether fraction (given oral; 100, 250 and 500 mg/kg) was established using the in vivo chemicals (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) models of nociception.The role of glutamate, TRPV1 receptor, bradykinin, protein kinase C, potassium channels, and various opioid and non-opioid receptors in modulating the partition's antinociceptive activity was also determined.The results obtained demonstrated that petroleum ether partition exerted significant (p < 0.05) antinociception in all the chemicals-, thermal-, capsaicin-, glutamate-, bradykinin, and phorbol 12-myristate 13-acetate (PMA)-induced nociception models.The antinociceptive activity was reversed following pretreatment with opioid antagonists (i.e.naloxone, β-funaltrexamine, naltrindole and nor-binaltorphimine), and the non-opioid receptor antagonists (i.e.pindolol (a β-adrenoceptor), haloperidol (a non-selective dopaminergic), atropine (a non-selective cholinergic receptor), caffeine (a non-selective adenosinergic receptor), and yohimbine (an α2-noradrenergic)).In addition, pretreatment with L-arginine (a nitric oxide (NO) donor), NG-nitro-L-arginine methyl esters (L-NAME; an inhibitor of NO synthase (NOS)), methylene blue (MB; an inhibitor of cyclic-guanosine monophosphate (cGMP) pathway), or their combination failed to inhibit petroleum ether partition's antinociception.In conclusion, petroleum ether partition exerts antinociceptive activity at the peripheral and central levels via the modulation of, partly, the opioid (i.e.µ, κ and δ) and several non-opioids (i.e.β-adrenergic, dopaminergic, cholinergic, adenosinergic, and α2-noradrenergic) receptors, glutamatergic, TRPV1 receptors, PKC and K+ channels systems, but not L-arg/NO/cGMP pathway.

LanguageEnglish
Pages408-419
Number of pages12
JournalBrazilian Journal of Pharmacognosy
Volume26
Issue number4
DOIs
StatePublished - 2016

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naphtha
Cyclic GMP
Bradykinin
Opioid Analgesics
Hot Temperature
TRPV1 receptor
naltrindole
beta-funaltrexamine
Pindolol
Nociception
Yohimbine
Narcotic Antagonists
Nitric Oxide Donors
Methylene Blue
Capsaicin
Potassium Channels
NG-Nitroarginine Methyl Ester
Glutamate Receptors
Cholinergic Receptors
Haloperidol

All Science Journal Classification (ASJC) codes

  • Pharmacology, Toxicology and Pharmaceutics(all)

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Antinociceptive effect of semi-purified petroleum ether partition of muntingia calabura leaves. / Zakaria, Zainul Amiruddin; Mohd Sani, Mohd Hijaz; Kadir, Arifah Abdul; Kek, Teh Lay; Salleh, Mohd Zaki.

In: Brazilian Journal of Pharmacognosy, Vol. 26, No. 4, 2016, p. 408-419.

Research output: Research - peer-reviewArticle

Zakaria, Zainul Amiruddin ; Mohd Sani, Mohd Hijaz ; Kadir, Arifah Abdul ; Kek, Teh Lay ; Salleh, Mohd Zaki. / Antinociceptive effect of semi-purified petroleum ether partition of muntingia calabura leaves. In: Brazilian Journal of Pharmacognosy. 2016 ; Vol. 26, No. 4. pp. 408-419
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abstract = "Muntingia calabura L., Muntingiaceae, is a medicinal plant for various pain-related diseases.The aims of the present study were to determine the antinociceptive profile and to elucidate the possible mechanisms of antinociception of petroleum ether partition obtained from crude methanol extract of M.calabura leaves using various animal models.The antinociceptive profile of petroleum ether fraction (given oral; 100, 250 and 500 mg/kg) was established using the in vivo chemicals (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) models of nociception.The role of glutamate, TRPV1 receptor, bradykinin, protein kinase C, potassium channels, and various opioid and non-opioid receptors in modulating the partition's antinociceptive activity was also determined.The results obtained demonstrated that petroleum ether partition exerted significant (p < 0.05) antinociception in all the chemicals-, thermal-, capsaicin-, glutamate-, bradykinin, and phorbol 12-myristate 13-acetate (PMA)-induced nociception models.The antinociceptive activity was reversed following pretreatment with opioid antagonists (i.e.naloxone, β-funaltrexamine, naltrindole and nor-binaltorphimine), and the non-opioid receptor antagonists (i.e.pindolol (a β-adrenoceptor), haloperidol (a non-selective dopaminergic), atropine (a non-selective cholinergic receptor), caffeine (a non-selective adenosinergic receptor), and yohimbine (an α2-noradrenergic)).In addition, pretreatment with L-arginine (a nitric oxide (NO) donor), NG-nitro-L-arginine methyl esters (L-NAME; an inhibitor of NO synthase (NOS)), methylene blue (MB; an inhibitor of cyclic-guanosine monophosphate (cGMP) pathway), or their combination failed to inhibit petroleum ether partition's antinociception.In conclusion, petroleum ether partition exerts antinociceptive activity at the peripheral and central levels via the modulation of, partly, the opioid (i.e.µ, κ and δ) and several non-opioids (i.e.β-adrenergic, dopaminergic, cholinergic, adenosinergic, and α2-noradrenergic) receptors, glutamatergic, TRPV1 receptors, PKC and K+ channels systems, but not L-arg/NO/cGMP pathway.",
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AU - Kek,Teh Lay

AU - Salleh,Mohd Zaki

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N2 - Muntingia calabura L., Muntingiaceae, is a medicinal plant for various pain-related diseases.The aims of the present study were to determine the antinociceptive profile and to elucidate the possible mechanisms of antinociception of petroleum ether partition obtained from crude methanol extract of M.calabura leaves using various animal models.The antinociceptive profile of petroleum ether fraction (given oral; 100, 250 and 500 mg/kg) was established using the in vivo chemicals (acetic acid-induced abdominal constriction and formalin-induced paw licking test) and thermal (hot plate test) models of nociception.The role of glutamate, TRPV1 receptor, bradykinin, protein kinase C, potassium channels, and various opioid and non-opioid receptors in modulating the partition's antinociceptive activity was also determined.The results obtained demonstrated that petroleum ether partition exerted significant (p < 0.05) antinociception in all the chemicals-, thermal-, capsaicin-, glutamate-, bradykinin, and phorbol 12-myristate 13-acetate (PMA)-induced nociception models.The antinociceptive activity was reversed following pretreatment with opioid antagonists (i.e.naloxone, β-funaltrexamine, naltrindole and nor-binaltorphimine), and the non-opioid receptor antagonists (i.e.pindolol (a β-adrenoceptor), haloperidol (a non-selective dopaminergic), atropine (a non-selective cholinergic receptor), caffeine (a non-selective adenosinergic receptor), and yohimbine (an α2-noradrenergic)).In addition, pretreatment with L-arginine (a nitric oxide (NO) donor), NG-nitro-L-arginine methyl esters (L-NAME; an inhibitor of NO synthase (NOS)), methylene blue (MB; an inhibitor of cyclic-guanosine monophosphate (cGMP) pathway), or their combination failed to inhibit petroleum ether partition's antinociception.In conclusion, petroleum ether partition exerts antinociceptive activity at the peripheral and central levels via the modulation of, partly, the opioid (i.e.µ, κ and δ) and several non-opioids (i.e.β-adrenergic, dopaminergic, cholinergic, adenosinergic, and α2-noradrenergic) receptors, glutamatergic, TRPV1 receptors, PKC and K+ channels systems, but not L-arg/NO/cGMP pathway.

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