Semipurified Ethyl Acetate Partition of Methanolic Extract of Melastoma malabathricum Leaves Exerts Gastroprotective Activity Partly via Its Antioxidant-Antisecretory-Anti-Inflammatory Action and Synergistic Action of Several Flavonoid-Based Compounds

Noor Wahida Ismail Suhaimy, Ahmad Khusairi Noor Azmi, Norhafizah Mohtarrudin, Maizatul Hasyima Omar, Siti Farah Md Tohid, Manraj Singh Cheema, Lay Kek Teh, Mohd Zaki Salleh, Zainul Amiruddin Zakaria

Research output: Research - peer-reviewArticle

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Abstract

Recent study has demonstrated the gastroprotective activity of crude methanolic extract of M. malabathricum leaves. The present study evaluated the gastroprotective potential of semipurified extracts (partitions): petroleum ether, ethyl acetate (EAMM), and aqueous obtained from the methanolic extract followed by the elucidation of the gastroprotective mechanisms of the most effective partition. Using the ethanol-induced gastric ulcer assay, all partitions exerted significant gastroprotection, with EAMM being the most effective partition. EAMM significantly (i) reduced the volume and acidity (free and total) while increasing the pH of gastric juice and enhanced the gastric wall mucus secretion when assessed using the pylorus ligation assay, (ii) increased the enzymatic and nonenzymatic antioxidant activity of the stomach tissue, (iii) lost its gastroprotective activity following pretreatment with N-omega-nitro-L-arginine methyl ester (L-NAME; NO blocker) or carbenoxolone (CBXN; NP-SH blocker), (iv) exerted antioxidant activity against various in vitro oxidation assays, and (v) showed moderate in vitro anti-inflammatory activity via the LOX-modulated pathway. In conclusion, EAMM exerts a remarkable NO/NP-SH-dependent gastroprotective effect that is attributed to its antisecretory and antioxidant activities, ability to stimulate the gastric mucus production and endogenous antioxidant system, and synergistic action of several gastroprotective-induced flavonoids.

LanguageEnglish
Article number6542631
JournalOxidative Medicine and Cellular Longevity
Volume2017
DOIs
StatePublished - 2017

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Flavonoids
Anti-Inflammatory Agents
Antioxidants
ethyl acetate
Stomach
Assays
NG-Nitroarginine Methyl Ester
Mucus
In Vitro Techniques
Carbenoxolone
Gastric Juice
Pylorus
Stomach Ulcer
Complex Mixtures
Ligation
Ethanol
naphtha
Acidity
Tissue
Oxidation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Ageing
  • Cell Biology

Cite this

Semipurified Ethyl Acetate Partition of Methanolic Extract of Melastoma malabathricum Leaves Exerts Gastroprotective Activity Partly via Its Antioxidant-Antisecretory-Anti-Inflammatory Action and Synergistic Action of Several Flavonoid-Based Compounds. / Ismail Suhaimy, Noor Wahida; Noor Azmi, Ahmad Khusairi; Mohtarrudin, Norhafizah; Omar, Maizatul Hasyima; Tohid, Siti Farah Md; Cheema, Manraj Singh; Teh, Lay Kek; Salleh, Mohd Zaki; Zakaria, Zainul Amiruddin.

In: Oxidative Medicine and Cellular Longevity, Vol. 2017, 6542631, 2017.

Research output: Research - peer-reviewArticle

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abstract = "Recent study has demonstrated the gastroprotective activity of crude methanolic extract of M. malabathricum leaves. The present study evaluated the gastroprotective potential of semipurified extracts (partitions): petroleum ether, ethyl acetate (EAMM), and aqueous obtained from the methanolic extract followed by the elucidation of the gastroprotective mechanisms of the most effective partition. Using the ethanol-induced gastric ulcer assay, all partitions exerted significant gastroprotection, with EAMM being the most effective partition. EAMM significantly (i) reduced the volume and acidity (free and total) while increasing the pH of gastric juice and enhanced the gastric wall mucus secretion when assessed using the pylorus ligation assay, (ii) increased the enzymatic and nonenzymatic antioxidant activity of the stomach tissue, (iii) lost its gastroprotective activity following pretreatment with N-omega-nitro-L-arginine methyl ester (L-NAME; NO blocker) or carbenoxolone (CBXN; NP-SH blocker), (iv) exerted antioxidant activity against various in vitro oxidation assays, and (v) showed moderate in vitro anti-inflammatory activity via the LOX-modulated pathway. In conclusion, EAMM exerts a remarkable NO/NP-SH-dependent gastroprotective effect that is attributed to its antisecretory and antioxidant activities, ability to stimulate the gastric mucus production and endogenous antioxidant system, and synergistic action of several gastroprotective-induced flavonoids.",
author = "{Ismail Suhaimy}, {Noor Wahida} and {Noor Azmi}, {Ahmad Khusairi} and Norhafizah Mohtarrudin and Omar, {Maizatul Hasyima} and Tohid, {Siti Farah Md} and Cheema, {Manraj Singh} and Teh, {Lay Kek} and Salleh, {Mohd Zaki} and Zakaria, {Zainul Amiruddin}",
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AU - Noor Azmi,Ahmad Khusairi

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AU - Omar,Maizatul Hasyima

AU - Tohid,Siti Farah Md

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AU - Salleh,Mohd Zaki

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